Royzen Lab | Research
Maksim (Max) Royzen research into applications of bio-orthogonal chemistry for in vivo imaging and RNA synthesis.
Maksim Royzen, Max Royzen, RNA imaging, live cell imaging, TCO, tetrazine, trans-cyclooctene, UAlbany Chemistry, RNA Institute, bio-orthogonal, bioorthogonal, MNP, nanoparticle, HMT, hydrogel, soft tissue sarcoma, STS, doxorubicin, dox-TCO, RNA-protein interactions, PRE, paramagnetic NMR, RNA synthesis, drug delivery
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RESEARCH

TOOLS FOR RNA RESEARCH

 

The Royzen Research Group is interested in developing new synthetic and imaging tools for RNA research. The interdisciplinary work on these projects involves chemical synthesis of nucleoside analogs, characterization of their photophysical properties, solid phase synthesis of RNA strands containing unnatural nucleosides and live cell imaging. Recently, we developed an in vitro transcription procedure for site-specific fluorescent labeling of RNA strands. In collaboration with Dharmacon, GE, we introduced a bio-orthogonal chemistry protocol for purification of synthetic RNA strands without RP HPLC. Lastly, we are developing fluorescent imaging probes of mRNA in live mammalian cells.

PARAMAGNETIC NMR PROBES

 

We are collaborating with Shekhtman Lab to develop transition metal-based NMR probes to study RNA-protein interactions in live cells using paramagnetic NMR techniques. The probes can be covalently attached to RNA in the vicinity of the protein’s binding site. These probes are capable of attenuating NMR signal intensities from protein residues localized in proximity to the paramagnetic probe as the result of RNA-protein interactions. The extent of the attenuation is related to the probe’s proximity, thus allowing us to construct the protein’s contact surface map. This new paradigm has been applied to study binding of HIV-1 nucleocapsid protein, NCp7, to a model RNA strands.

BIO-ORTHOGONAL CHEMISTRY

 

Together with an industrial collaborator, Shasqi, Inc. we are developing a bio-orthogonal chemistry-based approach termed ‘catch and release’, capable of concentrating and activating small molecules at a location of choice. The approach facilitates concentration of cytotoxic agents at the tumor site and, in contrast to existing targeted therapies, does not rely on endogenous cellular or environmental markers. The ‘catch and release’ strategy involves a biomaterial that is strategically implanted near a tumor. The biomaterial can react with systemically administered pro-drugs converting them into active cytotoxic agents leading to clear therapeutic benefits.